Core Facilities

Autosampler injects sample into an ultra high performance liquid chromatography (UPLC) for peptide separation and analysis by mass spectrometry

Bio-MS

We provide a mass spectrometry infrastructure for the identification, characterization, and absolute label free quantification of proteins and the targeted absolute quantification of small molecules in complex biological matrices. The core is a state-of-the-art mass spectrometry lab equipped with UPLC and 2D-nano-UPLC for LC-MS/MS and LC-MRM experiments. For protein and peptide separation and pre-fractionation we offer. [more...]

Selected publications

Hess, M.E., Hess, S., Meyer, K.D, Verhagen, L.A.W, Koch, L., Bronneke, H.S., Dietrich, M.O., Jordan, S.D., Saletore, Y., Elemento, O., Belgardt, B.F., Franz, T., Horvath, T.L., Ruther, U., Jaffrey, S.R., Kloppenburg, P., Bruning, J.C. (2013). The fat mass and obesity associated gene (Fto) regulates activity of the dopaminergic midbrain circuitry. Nature neuroscience 16 (8), 1042-1048.

Cámara, Y., Asin-Cayuela, J., Park, C.B., Metodiev, M.D., Shi, Y., Ruzzenente, B., Kukat, C., Habermann, B., Wibom, R., Hultenby, K., Franz, T., Erdjument-Bromage, H., Tempst, P., Hallberg, B.M., Gustafsson, C.M., Larsson, N.-G. (2011). MTERF4 Regulates Translation by Targeting the Methyltransferase NSUN4 to the Mammalian Mitochondrial Ribosome. Cell Metabolism 13, 527-539.

Reuter, M., Chuma, S., Tanaka, T., Franz, T., Stark, A., Pillai, R.S. (2009). Loss of the Mili-interacting Tudor domain-containing protein-1 activates transposons and alters the Mili-associated small RNA profile. Nat Struct Mol Biol. 16, 639-646.

Li, X., Wilm, M., Franz, T.  (2005). Silicone/Graphite coating for on-target desalting and improved peptide mapping performance of matrix-assisted laser desorption/ionization-mass spectrometry targets in proteomic experiments. Proteomics  5, 1460-1471.

Sola, M., Bavro, V.N., Timmins, J., Franz, T., Ricard-Blum, S., Schoehn, G., Ruigrok, R.W.H., Paarmann, I., Saiyed, T., O'Sullivan, G.A., Schmitt, B., Betz, H., Weissenhorn, W. (2004). Structural basis of dynamic glycine receptor clustering by gephyrin. EMBO Journal 23, 2510-2519.