CORE FACILITIES: FACS & IMAGING

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

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  • Chang, H. D.
  • Radbruch, A.
  • Acs, A.
  • Adam, D.
  • Adam-Klages, S.
  • Agace, W. W.
  • Aghaeepour, N.
  • Akdis, M.
  • Allez, M.
  • Almeida, L. N.
  • Alvisi, G.
  • Anderson, G.
  • Andra, I.
  • Annunziato, F.
  • Anselmo, A.
  • Bacher, P.
  • Baldari, C. T.
  • Bari, S.
  • Barnaba, V.
  • Barros-Martins, J.
  • Battistini, L.
  • Bauer, W.
  • Baumgart, S.
  • Baumgarth, N.
  • Baumjohann, D.
  • Baying, B.
  • Bebawy, M.
  • Becher, B.
  • Beisker, W.
  • Benes, V.
  • Beyaert, R.
  • Blanco, A.
  • Boardman, D. A.
  • Bogdan, C.
  • Borger, J. G.
  • Borsellino, G.
  • Boulais, P. E.
  • Bradford, J. A.
  • Brenner, D.
  • Brinkman, R. R.
  • Brooks, A. E. S.
  • Busch, D. H.
  • Buscher, M.
  • Bushnell, T. P.
  • Calzetti, F.
  • Cameron, G.
  • Cammarata, I.
  • Cao, X.
  • Cardell, S. L.
  • Casola, S.
  • Cassatella, M. A.
  • Cavani, A.
  • Celada, A.
  • Chatenoud, L.
  • Chattopadhyay, P. K.
  • Chow, S.
  • Christakou, E.
  • Cicin-Sain, L.
  • Clerici, M.
  • Colombo, F. S.
  • Cook, L.
  • Cooke, A.
  • Cooper, A. M.
  • Corbett, A. J.
  • Cosma, A.
  • Cosmi, L.
  • Coulie, P. G.
  • Cumano, A.
  • Cvetkovic, L.
  • Dang, V. D.
  • Dang-Heine, C.
  • Davey, M. S.
  • Davies, D.
  • De Biasi, S.
  • Del Zotto, G.
  • Dela Cruz, G. V.
  • Delacher, M.
  • Della Bella, S.
  • Dellabona, P.
  • Deniz, G.
  • Dessing, M.
  • Di Santo, J. P.
  • Diefenbach, A.
  • Dieli, F.
  • Dolf, A.
  • Dorner, T.
  • Dress, R. J.
  • Dudziak, D.
  • Dustin, M.
  • Dutertre, C. A.
  • Ebner, F.
  • Eckle, S. B. G.
  • Edinger, M.
  • Eede, P.
  • Ehrhardt, G. R. A.
  • Eich, M.
  • Engel, P.
  • Engelhardt, B.
  • Erdei, A.
  • Esser, C.
  • Everts, B.
  • Evrard, M.
  • Falk, C. S.
  • Fehniger, T. A.
  • Felipo-Benavent, M.
  • Ferry, H.
  • Feuerer, M.
  • Filby, A.
  • Filkor, K.
  • Fillatreau, S.
  • Follo, M.
  • Forster, I.
  • Foster, J.
  • Foulds, G. A.
  • Frehse, B.
  • Frenette, P. S.
  • Frischbutter, S.
  • Fritzsche, W.
  • Galbraith, D. W.
  • Gangaev, A.
  • Garbi, N.
  • Gaudilliere, B.
  • Gazzinelli, R. T.
  • Geginat, J.
  • Gerner, W.
  • Gherardin, N. A.
  • Ghoreschi, K.
  • Gibellini, L.
  • Ginhoux, F.
  • Goda, K.
  • Godfrey, D. I.
  • Goettlinger, C.
  • Gonzalez-Navajas, J. M.
  • Goodyear, C. S.
  • Gori, A.
  • Grogan, J. L.
  • Grummitt, D.
  • Grutzkau, A.
  • Haftmann, C.
  • Hahn, J.
  • Hammad, H.
  • Hammerling, G.
  • Hansmann, L.
  • Hansson, G.
  • Harpur, C. M.
  • Hartmann, S.
  • Hauser, A.
  • Hauser, A. E.
  • Haviland, D. L.
  • Hedley, D.
  • Hernandez, D. C.
  • Herrera, G.
  • Herrmann, M.
  • Hess, C.
  • Hofer, T.
  • Hoffmann, P.
  • Hogquist, K.
  • Holland, T.
  • Hollt, T.
  • Holmdahl, R.
  • Hombrink, P.
  • Houston, J. P.
  • Hoyer, B. F.
  • Huang, B.
  • Huang, F. P.
  • Huber, J. E.
  • Huehn, J.
  • Hundemer, M.
  • Hunter, C. A.
  • Hwang, W. Y. K.
  • Iannone, A.
  • Ingelfinger, F.
  • Ivison, S. M.
  • Jack, H. M.
  • Jani, P. K.
  • Javega, B.
  • Jonjic, S.
  • Kaiser, T.
  • Kalina, T.
  • Kamradt, T.
  • Kaufmann, S. H. E.
  • Keller, B.
  • Ketelaars, S. L. C.
  • Khalilnezhad, A.
  • Khan, S.
  • Kisielow, J.
  • Klenerman, P.
  • Knopf, J.
  • Koay, H. F.
  • Kobow, K.
  • Kolls, J. K.
  • Kong, W. T.
  • Kopf, M.
  • Korn, T.
  • Kriegsmann, K.
  • Kristyanto, H.
  • Kroneis, T.
  • Krueger, A.
  • Kuhne, J.
  • Kukat, C.
  • […]
  • Yazdanbakhsh, M.
  • Yu, L.
  • Yue, A.
  • Zhang, H.
  • Zhao, Y.
  • Ziegler, S. M.
  • Zielinski, C.
  • Zimmermann, J.
  • Zychlinsky, A.
(2019) Eur J Immunol, 49, 10, 1457-1973

Publikationen in denen die Core Facility FACS & Mikroskopie in den "Acknowledgments" aufgeführt wird

Schiffer I, Gerisch B, Kawamura K, Laboy R, Hewitt J, Denzel MS, Mori MA, Vanapalli S, Shen Y, Symmons O, Antebi A. (2021) miR-1 coordinately regulates lysosomal v-ATPase and biogenesis to impact proteotoxicity and muscle function during aging. Elife. 2021 Jul 27;10:e66768.

Kochan DZ, Mawer JSP, Massen J, Tishinov K, Parekh S, Graef M, Spang A, Tessarz P. (2021) The RNA-binding protein Puf5 contributes to buffering of mRNA upon chromatin-mediated changes in nascent transcription. J Cell Sci. 2021 Jul 14:jcs.259051.

Koester J, Miroshnikova YA, Ghatak S, Chacón-Martínez CA, Morgner J, Li X, Atanassov I, Altmüller J, Birk DE, Koch M, Bloch W, Bartusel M, Niessen CM, Rada-Iglesias A, Wickström SA. (2021) Niche stiffening compromises hair follicle stem cell potential during ageing by reducing bivalent promoter accessibility. Nat Cell Biol. 2021 Jul 8.

Jiang M, Xie X, Zhu X, Jiang S, Milenkovic D, Misic J, Shi Y, Tandukar N, Li X, Atanassov I, Jenninger L, Hoberg E, Albarran-Gutierrez S, Szilagyi Z, Macao B, Siira SJ, Carelli V, Griffith JD, Gustafsson CM, Nicholls TJ, Filipovska A, Larsson NG, Falkenberg M. (2021) The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication. Sci Adv. 2021 Jul 2;7(27):eabf8631.

Miroshnikova YA, Manet S, Li X, Wickström SA, Faurobert E, Albiges-Rizo C (2021) Calcium signaling mediates a biphasic mechanoadaptive response of endothelial cells to cyclic mechanical stretch. Mol Biol Cell. 2021 Jun 3

Lu YX, Regan JC, Eßer J, Drews LF, Weinseis T, Stinn J, Hahn O, Miller RA, Grönke S, Partridge L (2021) A TORC1-histone axis regulates chromatin organisation and non-canonical induction of autophagy to ameliorate ageing.Elife. 2021 May 14

Behrends J, Schwager C, Hein M, Scholzen T, Kull S, Jappe U. (2021) Innovative robust basophil activation test using a novel gating strategy reliably diagnosing allergy with full automation. Allergy. 2021 May 11.

Allmeroth K, Kim CS, Annibal A, Pouikli A, Koester J, Derisbourg MJ, Andrés Chacón-Martínez C, Latza C, Antebi A, Tessarz P, Wickström SA, Denzel MS (2021) N1-acetylspermidine is a determinant of hair follicle stem cell fate.J Cell Sci. 2021 May 1;134(9)

Sprenger HG, MacVicar T, Bahat A, Fiedler KU, Hermans S, Ehrentraut D, Ried K, Milenkovic D, Bonekamp N, Larsson NG, Nolte H, Giavalisco P, Langer T. (2021) Cellular pyrimidine imbalance triggers mitochondrial DNA-dependent innate immunity. Nat Metab. 2021 Apr 26.

Kroef V, Ruegenberg S, Horn M, Allmeroth K, Ebert L, Bozkus S, Miethe S, Schermer B, Baumann U, Denzel MS (2021) GFAT2 and AMDHD2 act in tandem to control the hexosamine biosynthetic pathway.  bioRxiv 2021.04.23.441115

Tain LS, Sehlke R, Meilenbrock RL, Leech T, Paulitz J, Chokkalingam M, Nagaraj N, Grönke S, Fröhlich J, Atanassov I, Mann M, Beyer A, Partridge L. (2021) Tissue-specific modulation of gene expression in response to lowered insulin signalling in Drosophila.  Elife. 2021 Apr 21;10:e67275.

L Dolfi, TK Suen, R Ripa, A Antebi (2021) Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri.Research Square

Schiffer I, Gerisch B, Kawamura K, Laboy R, Hewitt J, Denzel MS, Mori MA, Vanapalli S, Shen Y, Symmons O, Antebi A (2021) miR-1 coordinately regulates lysosomal v-ATPase and biogenesis to affect muscle contractility upon proteotoxic challenge during ageing.  bioRxiv 2021.01.21.427623

Lu J, Temp U, Müller-Hartmann A, Esser J, Grönke S, Partridge L (2021) Sestrin is a key regulator of stem cell function and lifespan in response to dietary amino acids.  Nature Aging 1, 60–72 (2021)

Bonfiglio JJ, Leidecker O, Dauben H, Longarini EJ, Colby T, San Segundo-Acosta P, Perez KA, Matic I. (2020) An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation.Cell. 2020 Nov 12;183(4):1086-1102.e23

Motori E, Atanassov I, Kochan SMV, Folz-Donahue K, Sakthivelu V, Giavalisco P, Toni N, Puyal J, Larsson NG. (2020) Neuronal metabolic rewiring promotes resilience to neurodegeneration caused by mitochondrial dysfunction.Sci Adv. 2020 Aug 28;6(35):eaba8271.

Kim CS, Ding X, Allmeroth K, Biggs LC, Kolenc OI, L'Hoest N, Chacón-Martínez CA, Edlich-Muth C, Giavalisco P, Quinn KP, Denzel MS, Eming SA, Wickström SA. (2020) Glutamine Metabolism Controls Stem Cell Fate Reversibility and Long-Term Maintenance in the Hair Follicle.Cell Metab. 2020 Oct 6;32(4):629-642.e8.

Cui D, Mesaros A, Burdeos G, Voigt I, Giavalisco P, Hinze Y, Purrio M, Neumaier B, Drzezga A, Obata Y, Endepols H, Xu X. (2020) Dnmt3a2/Dnmt3L Overexpression in the Dopaminergic System of Mice Increases Exercise Behavior through Signaling Changes in the Hypothalamus.Int J Mol Sci. 2020 Aug 31;21(17):6297.

Steven P, Schwab S, Kiesewetter A, Saban DR, Stern ME, Gehlsen U. (2020) Disease-Specific Expression of Conjunctiva Associated Lymphoid Tissue (CALT) in Mouse Models of Dry Eye Disease and Ocular Allergy. Int J Mol Sci. 2020 Oct 12;21(20):E7514.

Kochan DZ, Mawer JSP, Tishinov K, Parekh S, Massen J, Graef M, Spang A, Tessarz P (2020) The RNA-binding protein Puf5 buffers mRNA levels against chromatin-mediated changes in nascent transcription. bioRxiv 10.1101/2020.08.13.249912

Dolfi L, Suen TK, Ripa R, Antebi A (2020) Optimization of cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri. bioRxiv 10.1101/2020.04.14.040824

Allmeroth K, Horn M, Kroef V, Miethe S, Müller RU, Denzel MS. (2020) Bortezomib resistance mutations in PSMB5 determine response to second-generation proteasome inhibitors in multiple myeloma. Leukemia. 2020 Jul 20.

Damen M, Soroka E, Khatif H, Kukat C, Simons BD, Bazzi H (2020) Epidermal stratification is uncoupled from centrosome-dependent cell division orientation of the basal progenitors.bioRxiv 10.1101/2020.07.24.219493.   

Alsina D, Lytovchenko O, Schab A, Atanassov I, Schober FA, Jiang M, Koolmeister C, Wedell A, Taylor RW, Wredenberg A, Larsson NG. (2020) FBXL4 deficiency increases mitochondrial removal by autophagy.EMBO Mol Med. 2020 Jul 7;12(7):e11659.

Tharyan RG, Annibal A, Schiffer I, Laboy R, Atanassov I, Weber AL, Gerisch B, Antebi A. (2020) NFYB-1 regulates mitochondrial function and longevity via lysosomal prosaposin.Nat Metab. 2020 May;2(5):387-396

Gerisch, B., Tharyan, R. G., Mak, J., Denzel, S. I., Popkes-van Oepen, T., Henn, N., & Antebi, A. (2020). HLH-30/TFEB Is a Master Regulator of Reproductive Quiescence. Developmental cell, 53(3), 316–329.e5.

Nava, M. M., Miroshnikova, Y. A., Biggs, L. C., Whitefield, D. B., Metge, F., Boucas, J., Vihinen, H., Jokitalo, E., Li, X., García Arcos, J. M., Hoffmann, B., Merkel, R., Niessen, C. M., Dahl, K. N., & Wickström, S. A. (2020). Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage.Cell, 181(4), 800–817.e22.

Ruegenberg, S., Horn, M., Pichlo, C., Allmeroth, K., Baumann, U., & Denzel, M. S. (2020). Loss of GFAT-1 feedback regulation activates the hexosamine pathway that modulates protein homeostasis.Nature communications, 11(1), 687.

Schütter, M., Giavalisco, P., Brodesser, S., & Graef, M. (2020). Local Fatty Acid Channeling into Phospholipid Synthesis Drives Phagophore Expansion during Autophagy. Cell, 180(1), 135–149.e14.

Morón-Oset, J., Supèr, T., Esser, J., Isaacs, A. M., Grönke, S., & Partridge, L. (2019). Glycine-alanine dipeptide repeats spread rapidly in a repeat length- and age-dependent manner in the fly brain.Acta neuropathologica communications, 7(1), 209.

Dolfi, L., Ripa, R., Antebi, A., Valenzano, D. R., & Cellerino, A. (2019). Cell cycle dynamics during diapause entry and exit in an annual killifish revealed by FUCCI technology.EvoDevo, 10, 29.

Tain, L. S., Jain, C., Nespital, T., Froehlich, J., Hinze, Y., Grönke, S., & Partridge, L. (2020). Longevity in response to lowered insulin signaling requires glycine N-methyltransferase-dependent spermidine production.Aging cell, 19(1), e13043.

Busch, J. D., Cipullo, M., Atanassov, I., Bratic, A., Silva Ramos, E., Schöndorf, T., Li, X., Pearce, S. F., Milenkovic, D., Rorbach, J., & Larsson, N. G. (2019). MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.Cell reports, 29(6), 1728–1738.e9

Guerrero GA, Schilling K, Mayr FAMC, Lu R, Benayoun BA, Denzel MS (2019) NHR-8 regulated P-glycoproteins uncouple xenobiotic stress resistance from longevity in chemosensory C. elegans mutants.bioRxiv 823302

Korzelius, J., Azami, S., Ronnen-Oron, T., Koch, P., Baldauf, M., Meier, E., Rodriguez-Fernandez, I. A., Groth, M., Sousa-Victor, P., & Jasper, H. (2019). The WT1-like transcription factor Klumpfuss maintains lineage commitment of enterocyte progenitors in the Drosophila intestine.Nature communications, 10(1), 4123.

Silva Ramos E, Motori E, Brüser C, Kühl I, Yeroslaviz A, Ruzzenente B, Kauppila JHK, Busch JD, Hultenby K, Habermann BH, Jakobs S, Larsson NG, Mourier A. (2019) Mitochondrial fusion is required for regulation of mitochondrial DNA replication. PLoS Genet. 2019 Jun 6;15(6):e1008085.

Weigelt CM, Hahn O, Arlt K, Gruhn M, Jahn AJ, Eßer J, Werner JA, Klein C, Büschges A, Grönke S, Partridge L. (2019) Loss of miR-210 leads to progressive retinal degeneration in Drosophila melanogaster.Life Sci Alliance. 2019 Jan 22;2(1). pii: e201800149.

Mylonas C, Tessarz P (2018) Transcriptional repression by FACT is linked to regulation of chromatin accessibility at the promoter of ES cells.Life Sci Alliance. 2018 Jun 13;1(3):e201800085.

Kauppila JHK, Bonekamp NA, Mourier A, Isokallio MA, Just A, Kauppila TES, Stewart JB, Larsson NG (2018) Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice.Nucleic Acids Res. 2018 Jul 27;46(13):6642-6669.

Simard ML, Mourier A, Greaves LC, Taylor RW, Stewart JB (2018) A novel histochemistry assay to assess and quantify focal cytochrome c oxidase deficiency.J Pathol. 2018 Jul;245(3):311-323.

Mo D, Cui D, Li X (2018) The role of Aß circRNA in Alzheimer’s disease: alternative mechanism of Aß biogenesis from Aß circRNA translation.bioRxiv

Medeiros TC, Thomas RL, Ghillebert R, Graef M (2018) Autophagy balances mtDNA synthesis and degradation by DNA polymerase POLG during starvation.J Cell Biol. 2018 May 7;217(5):1601-1611.

Horn M, Kroef V, Allmeroth K, Schuller N, Miethe S, Peifer M, Elling U, Penninger JM,  Denzel MS (2018) Unbiased compound-protein interface mapping and prediction of chemoresistance loci through forward genetics in haploid stem cells.Oncotarget January 23, 2018

Miroshnikova YA, Le HQ, Schneider D, Thalheim T, Rübsam M, Bremicker N, Polleux J, Kamprad N, Tarantola M, Wang I, Balland M, Niessen CM, Galle J, Wickström SA (2017) Adhesion forces and cortical tension couple cell proliferation and differentiation to drive epidermal stratification.Nat Cell Biol. 2017

Kohli, P., Höhne, M., Jüngst, C., Bertsch, S., Ebert, L. K., Schauss, A. C., Benzing, T., Rinschen, M. M., & Schermer, B. (2017). The ciliary membrane-associated proteome reveals actin-binding proteins as key components of cilia.EMBO reports, 18(9), 1521–1535.

Gorsky MK, Burnouf S, Sofola-Adesakin O, Dols J, Augustin H, Weigelt CM, Grönke S, Partridge L (2017) Pseudo-acetylation of multiple sites on human Tau proteins alters Tau phosphorylation and microtubule binding, and ameliorates amyloid beta toxicity.Sci Rep. 2017 Aug 30;7(1):9984.

Jiang M, Kauppila TES, Motori E, Li X, Atanassov I, Folz-Donahue K, Bonekamp NA, Albarran-Gutierrez S, Stewart JB, Larsson NG (2017) Increased Total mtDNA Copy Number Cures Male Infertility Despite Unaltered mtDNA Mutation Load.Cell Metab. 2017 Aug 1;26(2):429-436.e4.

Kohli P, Höhne M, Jüngst C, Bertsch S, Ebert LK, Schauss AC, Benzing T, Rinschen MM, Schermer B (2017) The ciliary membrane-associated proteome reveals actin-binding proteins as key components of cilia.EMBO Rep. 2017 Jul 14. pii: e201643846.

Theurich S, Tsaousidou E, Hanssen R, Lempradl AM, Mauer J, Timper K, Schilbach K, Folz-Donahue K, Heilinger C, Sexl V, Pospisilik JA, Wunderlich FT, Brüning JC (2017) IL-6/Stat3-Dependent Induction of a Distinct, Obesity-Associated NK Cell Subpopulation Deteriorates Energy and Glucose Homeostasis.Cell Metab. 2017 Jul 5;26(1):171-184.e6.

Chacón-Martínez CA, Klose M, Niemann C, Glauche I, Wickström SA (2016) Hair follicle stem cell cultures reveal self-organizing plasticity of stem cells and their progeny.EMBO J. 2017 Jan 17;36(2):151-164.

Kauppila JH, Baines HL, Bratic A, Simard ML, Freyer C, Mourier A, Stamp C, Filograna R, Larsson NG, Greaves LC, Stewart JB (2016) A Phenotype-Driven Approach to Generate Mouse Models with Pathogenic mtDNA Mutations Causing Mitochondrial Disease. Cell Rep. 2016 Sep 13;16(11):2980-2990.

Kühl I, Miranda M, Posse V, Milenkovic D, Mourier A, Siira SJ, Bonekamp NA, Neumann U, Filipovska A, Polosa PL, Gustafsson CM, Larsson NG (2016) POLRMT regulates the switch between replication primer formation and gene expression of mammalian mtDNA. Sci Adv. 2016 Aug 5;2(8):e1600963.

Le HQ, Ghatak S, Yeung CC, Tellkamp F, Günschmann C, Dieterich C, Yeroslaviz A, Habermann B, Pombo A, Niessen CM, Wickström SA (2016) 
Mechanical regulation of transcription controls Polycomb-mediated gene silencing during lineage commitment. Nat Cell Biol. 2016 Aug;18(8):864-75.

Bratic A, Clemente P, Calvo-Garrido J, Maffezzini C, Felser A, Wibom R, Wedell A, Freyer C, Wredenberg A (2016) Mitochondrial Polyadenylation Is a One-Step Process Required for mRNA Integrity and tRNA MaturationPLoS Genet. 2016 May 13;12(5):e1006028.

Nakamura S, Karalay Ö, Jäger PS, Horikawa M, Klein C, Nakamura K, Latza C, Templer SE, Dieterich C, Antebi A (2016) Mondo complexes regulate TFEB via TOR inhibition to promote longevity in response to gonadal signals. Nat Commun 7:10944.

Velázquez AP, Tatsuta T, Ghillebert R, Drescher I, Graef M (2016) Lipid droplet-mediated ER homeostasis regulates autophagy and cell survival during starvation. J Cell Biol 212(6):621-31.

Morgner J, Ghatak S, Jakobi T, Dieterich C, Aumailley M, Wickström SA (2015) Integrin-linked kinase regulates the niche of quiescent epidermal stem cells. Nat Commun 6:8198.

Mourier A, Motori E, Brandt T, Lagouge M, Atanassov I, Galinier A, Rappl G, Brodesser S, Hultenby K, Dieterich C, Larsson NG (2015) Mitofusin 2 is required to maintain mitochondrial coenzyme Q levels. J Cell Biol 208, 429-442.

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