The CRISPR screening facility provides access to functional genomic screening using CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat). The CRISPR-Cas9 system is composed of two components – the CRISPR-associated endonuclease Cas9 and a single guide RNA (sgRNA), which directs Cas9 to genomic loci in order to enable perturbations, such as knock-outs. Screening with CRISPR-Cas9 is able to generate powerful and robust data to e.g. identify novel biology hits or provide insights into the mechanisms of action of drugs, toxins or pathogens.
Screening can be performed with bespoke custom libraries all the way up to whole genome using gold-standard genome-wide libraries. The main goal of the facility is to provide access to CRISPR-Cas9 screening without the need to establish the technique or generate reagents. Therefore, the facility holds state-of-the-art pooled libraries for CRISPRko, CRISPRi or CRISPRa screening and provides guidance throughout the entire screening process –evaluation of cell lines and screen system, screen assay, readout and data analysis.