CORE FACILITIES: FACS & IMAGING

Flow Cytometry is a technology that simultaneously measures and analyzes multiple physical characteristics of single cells or other particles as they move in a stream of liquid and pass a beam of light.

Flow cytometers:

• BD FACSAria IIIu
  High-speed cell sorter and analyzer with 4 lasers and 14 fluorescent parameters.

• BD FACSAria Fusion
  High-speed cell sorter and analyzer with 5 lasers and 18 fluorescent parameters and biosafety cabinet.

• BD FACSCanto II
  Analyzer with 3 lasers and 8 fluorescent parameters.

• Miltenyi autoMACS Pro
  Magnetic cell separator.

Microscopy is the technology of using microscopes to view samples and objects that cannot be seen with the unaided eye.

Widefield microscopes:

• Nikon Eclipse Ci
  Upright microscope for bright field and polarized light microscopy

• Laser-scanning confocal Leica SP8-X
  Inverted laser-scanning confocal microscope with different lasers and 5 fluorescent detectors. Equipped with incubation chamber for live-cell imaging.

• Spinning disc confocal Andor Dragonfly
  Inverted spinning disc microscope with 4 lasers and 2 cameras. Equipped with incubation chamber for live-cell imaging.

• Multiphoton microscope Leica SP8-MP-OPO
  Upright laser-scanning microscope with multiphoton lasers (up to 1280 nm) and 7 fluorescent detectors.

The histology unit hosts equipment for the study of the microscopic anatomy of cells and tissues including instruments for fixing, processing, embedding, sectioning, and staining.

• Tissue processor
• Heated paraffin embedding module
• Microtome
• Cryostat
• Vibratome
• Antigen Retriever
• Automated Slide Stainer
• Cassette and slide printer
• Tissue Microarrayer
• Batch stainer
• Microscope