DEFACS & Imaging Core Facility
SERVICES
The FACS & Imaging Core Facility is home to 30 bookable resources, including wide-field and confocal microscopes, flow cytometry analyzers and sorters, shared histology equipment, and PC workstations for data analysis.
We provide additional services such as software training, access to analysis software, and help with experimental planning and post-acquisition analysis.
INSTRUMENTS
Flow cytometry (FACS)
Flow Cytometry is a technology that simultaneously measures and analyzes multiple physical characteristics of single cells or other particles as they move in a stream of liquid and pass a beam of light.
Flow cytometers:
- BD FACSAria IIIu
High-speed cell sorter and analyzer with 4 lasers and 14 fluorescent parameters.
- BD FACSAria Fusion
High-speed cell sorter and analyzer with 5 lasers and 18 fluorescent parameters and biosafety cabinet.
- BD FACSCanto II
Analyzer with 3 lasers and 8 fluorescent parameters.
- BD LSRFortessa
Analyzer with 5 lasers and 18 fluorescent parameters.
- Miltenyi autoMACS Pro
Magnetic cell separator.
Microscopy (Imaging)
Microscopy is the technology of using microscopes to view samples and objects that cannot be seen with the unaided eye.
Widefield microscopes:
- Nikon Eclipse Ci
Upright microscope for bright field and polarized light microscopy
- Leica DMI6000B
Inverted fluorescent microscope, equipped with incubation chamber for live-cell imaging.
- EssenBioscience IncuCyte S3
Live cell imaging and fast screening system.
- ThermoFisher EVOS FL Auto 2
Inverted fluorescent microscope, equipped with on-stage incubator for live-cell imaging.
Confocal Microscopes:
- Laser-scanning confocal SP8-DLS
Inverted laser-scanning confocal microscope with different lasers and 5 fluorescent detectors. Equipped with incubation chamber for live-cell imaging. It can be used as a light-sheet microscope.
- Laser-scanning confocal Leica SP8-X
Inverted laser-scanning confocal microscope with different lasers and 5 fluorescent detectors. Equipped with incubation chamber for live-cell imaging.
- Spinning disc confocal Olympus SpinSR
Inverted spinning disc microscope with 6 lasers and 2 cameras. Equipped with incubation chamber for live-cell imaging.
- Spinning disc confocal Andor Dragonfly
Inverted spinning disc microscope with 4 lasers and 2 cameras. Equipped with incubation chamber for live-cell imaging.
- Laser-scanning confocal Leica SP8-Upright
Upright laser-scanning confocal microscope with different lasers and 5 fluorescent detectors.
- Superresolution microscope Abberior Instruments Facility Line
Superresolution stimulated-emission depletion (STED) microscope with 8 lasers and 4 fluorescent detectors.
Histology Lab
The histology unit hosts equipment for the study of the microscopic anatomy of cells and tissues including instruments for fixing, processing, embedding, sectioning, and staining.
- Tissue processor
- Heated paraffin embedding module
- Microtome
- Cryostat
- Vibratome
- Antigen Retriever
- Automated Slide Stainer
- Cassette and slide printer
- Tissue Microarrayer
- Batch stainer
- Microscope