CORE FACILITIES: FACS & IMAGING

The FACS & Imaging Core Facility is home to 31 bookable resources, including wide-field and confocal microscopes, flow cytometry analyzers and sorters, shared histology equipment, and PC workstations for data analysis.

We provide additional services such as software training, access to analysis software, and help with experimental planning and post-acquisition analysis.

INSTRUMENTS

Flow Cytometry is a technology that simultaneously measures and analyzes multiple physical characteristics of single cells or other particles as they move in a stream of liquid and pass a beam of light.

Flow cytometers:

• BD FACSAria IIIu
  High-speed cell sorter and analyzer with 4 lasers and 14 fluorescent parameters.

• BD FACSAria Fusion
  High-speed cell sorter and analyzer with 5 lasers and 18 fluorescent parameters and biosafety cabinet.

• BD FACSCanto II
  Analyzer with 3 lasers and 8 fluorescent parameters.

• Miltenyi MACSQuant VYB
  Analyzer with 3 lasers and 8 fluorescent parameters. Integrated with Thermo Scientific Cytomat plate hotel.

• Miltenyi autoMACS Pro
  Magnetic cell separator.

Microscopy is the technology of using microscopes to view samples and objects that cannot be seen with the unaided eye.

Widefield microscopes:

• Nikon Eclipse Ci
  Upright microscope for bright field and polarized light microscopy

• Laser-scanning confocal Leica SP8-X
  Inverted laser-scanning confocal microscope with different lasers and 5 fluorescent detectors. Equipped with incubation chamber for live-cell imaging.

• Spinning disc confocal Visitron CSU X1
  Inverted spinning disc microscope with 4 lasers and 3 cameras. Equipped with incubation chamber for live-cell imaging.

• Multiphoton microscope Leica SP8-MP-OPO
  Upright laser-scanning microscope with multiphoton lasers (up to 1280 nm) and 7 fluorescent detectors.

The histology unit hosts equipment for the study of the microscopic anatomy of cells and tissues including instruments for fixing, processing, embedding, sectioning, and staining.

• Tissue processor

• Heated paraffin embedding module

• Microtome

• Cryostat

• Vibratome

• Antigen Retriever

• Batch stainer

• Microscope