CORE FACILITIES: FACS & IMAGING

Services

The FACS & Imaging Core Facility is home to 24 bookable resources, including wide-field and confocal microscopes, flow cytometry analyzers and sorters, shared histology equipment, and PC workstations for data analysis.

We provide additional services such as software training, access to analysis software, and help with experimental planning and post-acquisition analysis.

INSTRUMENTS

Microscopy (Imaging)

Microscopy is the technology of using microscopes to view samples and objects that cannot be seen with the unaided eye.

Widefield microscopes:

  • Nikon Eclipse Ci
    Upright microscope for bright field and polarized light microscopy

  • Leica DMI3000B
    Inverted fluorescent microscope with 2 cameras.

  • NanoEnTek JuLI Br
    Live cell movie analyzer for live-cell imaging in parallel.

Confocal Microscopes:

  • Laser-scanning confocal Leica SP5-X
    Inverted laser-scanning confocal microscope with different lasers and 5 fluorescent detectors. Equipped with incubation chamber for live-cell imaging.

 

 

 

  • Laser-scanning confocal Leica SP8-X
    Inverted laser-scanning confocal microscope with different lasers and 5 fluorescent detectors. Equipped with incubation chamber for live-cell imaging.

  • Spinning disc confocal Visitron CSU X1
    Inverted spinning disc microscope with 4 lasers and 3 cameras. Equipped with incubation chamber for live-cell imaging.

  • Multiphoton microscope Leica SP8-MP-OPO
    Upright laser-scanning microscope with multiphoton lasers (up to 1280 nm) and 7 fluorescent detectors.

Flow cytometry (FACS)

Flow Cytometry is a technology that simultaneously measures and analyzes multiple physical characteristics of single cells or other particles as they move in a stream of liquid and pass a beam of light.

Flow cytometers:

  • BD FACSAria Fusion
    High-speed cell sorter and analyzer with 5 lasers and 18 fluorescent parameters.

  • BD FACSAria IIIu
    High-speed cell sorter and analyzer with 4 lasers and 14 fluorescent parameters.

  • BD FACSCanto II
    Analyzer with 3 lasers and 8 fluorescent parameters.

  • Miltenyi MACSQuant VYB
    Analyzer with 3 lasers and 8 fluorescent parameters. Integrated with Thermo Scientific Cytomat Plate Hotel and Orbitor Microplate Mover.

  • Miltenyi autoMACS Pro
    Magnetic cell separator.

Histology Lab

The histology unit hosts equipment for the study of the microscopic anatomy of cells and tissues including instruments for fixing, processing, embedding, sectioning, and staining.

  • Tissue processor

  • Heated paraffin embedding module

  • Microtome

  • Cryostat

  • Vibratome

  • Antigen Retriever

  • Batch stainer

  • Microscope