Stem cells feel the force

If cells are stretched or compressed, their genetic material changes

Stem cells feel and respond to external mechanical forces. This finding was reported by a research group led by Sara Wickström, Max Planck Research Group Leader at the Max Planck Institute for Biology of Ageing, Cologne. External stimuli They do this by changing the way DNA is organized in the nucleus, and thereby the activity of genes that are required for stem cell differentiation. 

All cells share the same genetic code, no matter if they are skin or brain cells. However, these cells are exposed to very different types of mechanical environments and mechanical stresses. For example, brain tissue is very soft, whereas bone is hard. Researchers know that cells respond to extrinsic forces by changing their structure and their gene activity to be better suited for their particular environments and to be able to execute their specific functions. The molecular mechanisms of this regulation are, however, not yet clear

“Our skin protects us against the outside world while being constantly exposed to toxic insults, injuries, UV radiation and mechanical strain. Therefore it is particularly important for skin cells to be able to respond to forces”, says Huy Quang Le, the leading scientist of the study. 

Streched stem cells

To study how skin cells respond to forces, Le and his colleagues used a special mechanical device, which stretched skin stem cell cultures similar to what they would experience inside the tissues. The researchers analysed the gene activity of these mechanically stretched stem cells using next generation sequencing. The results revealed that thousands of genes were downregulated, whereas very few genes increased their expression. Further research revealed that stretch induced global changes in how DNA is packed within the nucleus. As a result less DNA is transcribed. 

For a stem cell to differentiate, it needs to transcribe a large number of genes to acquire its specialized architecture and function. As a result of the mechanical strain, stretched stem cells would not differentiate in the presence of a differentiation signal. “It was exciting to realize that we could alter the structural organization of DNA simply by exerting mechanical forces on the stem cells”, says Sara Wickström. 

Going deeper into the cellular mechanism of the DNA rearrangements, Le and his colleagues found out that the mechanical forces were being sensed at the nuclear envelope, a structure that surrounds the DNA and separates it from the rest of the cell. A key molecule in this force sensing was a protein called emerin, which links the nucleus and DNA to the skeleton of the cell. 

Emery-Dreifus muscular dystrophy

Emerin can occur in a mutated form in a disease called Emery-Dreifus muscular dystrophy. Patients suffer from a degeneration of mechanically strained tissues such as the skeletal muscle, heart, and also skin. “As the precise pathomechanisms of this disease are unknown and we lack efficient treatment, a major future goal of the laboratory is to understand whether the mechanisms uncovered in this study play a role in the development of the disease”, says Sara Wickström. As the mechanical properties of tissues change with age, the researchers further want to understand how aged stem cells sense forces and how the altered mechanical properties of the surrounding tissue would alter this.

Sara Wickström’s research group is one of three Paul Gerson Unna Research Groups within the Max-Planck-Society, kindly funded by the Max und Ingeburg Herz-Foundation with the support of the Max-Planck-Foundation. Sara Wickström is also Principal Investigator at CECAD, the Cluster of Excellence in Cellular Stress Responses in Aging-associated Diseases at the University of Cologne.

MB/HR