Proteomics and ADP-ribosylation signaling
The overall aim of our research group is to understand the molecular mechanisms of DNA damage response and ageing by elucidating the role of ADP-ribosylation in these biological processes.
Reversible post-translational modifications (PTMs) of proteins control a myriad of key physiological and pathological processes. ADP-ribosylation (ADPr), a PTM of major interest for the research group of Ivan Matic, involves adding adenosine diphosphate ribose molecules to target proteins, modulating their function at different levels, such as subcellular localization, stability and activity. ADPr is mainly known as the signalling molecule catalysed by PARP-1, a much-studied enzyme that plays a critical role in DNA repair by ADP-ribosylating and recruiting essential chromatin factors, including histones and proteins essential for DNA repair. Despite a wealth of evidence pointing to the biomedical importance of PARP-1 signalling especially in DNA damage responses and cancer therapy, a deeper understanding of the processes regulated by ADPr has been hampered by difficulties in establishing the underlying molecular mechanisms and the exact conjugation chemistry.
*equal contribution; **corresponding author
- 2021 - 2026 | ERC Consolidator Grant
- 2019 - 2023 | EMBO Young Investigator Program