CORE FACILITIES: PROTEOMICS

The mission of the Proteomics Core Facility is to provide an analytic platform and to couple it to users' research interests. The core facility is a state-of-the-art mass spectrometry lab equipped with top-level instrumentation, complemented by expertise ranging from sample preparation strategies, through mass spectrometry (MS) to computational proteomics. Given the highly technological nature of this approach, the facility's primary focus is on analytic instrumentation and methodology. However, to bring the technology closer to scientific users, we also focus on instruction, consultation and discussion at every stage of a project. Close attention is given to qualityservice, fast reaction times to users' requests and the complete integration of the facility with the scientific objectives of the institute.

  • Protein digestion using a variety of proteases (trypsin, Lys-C, Glu-C, Asp-N etc.) and approaches (in solution, FASP, in gel)

  • StageTips (C18, SAX, SCX, SDB-RPS, SDB-XC): purification, desalting, fractionation and concentration

  • gel electrophoresis, staining and imaging (Gel Doc EZ imager)

Orbitrap Fusion Tribrid ETD with EASY 1000 UPLC

Q Exactive HF with EASY 1000 UPLC

Waters Xevo Triple Quadrupole with Acquity UPLC

  • MaxQuant quantitative proteomics software package

  • Perseus framework

  • Statistical environment R

  • Mascot

  • ProteinScape