Services
Proteomics Core Facility
Quantitative proteomics
- Tandem Mass Tag (TMT) quantification strategy coupled with high-pH peptide fractionation for maximal analytical depth
- Interaction proteomics by label-free quantification and Data-dependent acquisitions (DDA)
- Label-free Data-Independent Acquisition (DIA) for protein quantitation for large sample cohorts
Sample preparation
- Protein digestion using a variety of proteases (trypsin, Lys-C, Glu-C, Asp-N etc.) and approaches (in solution, FASP, in gel)
- StageTips (C18, SAX, SCX, SDB-RPS, SDB-XC): purification, desalting, fractionation and concentration
- Off-line Pre-fractionating via HPLC
- gel electrophoresis, staining and imaging (Gel Doc EZ imager)
- TMT labeling
- SP3 sample preparation for detergent-containing samples
Mass spectrometry
Orbitrap Fusion Lumos Tribrid ETD with EASY 1200 UPLC and FAIMS Pro Interface
Orbitrap Fusion Tribrid ETD with EASY 1000 UPLC
Q Exactive HF with EASY 1000 UPLC
timsTOF Pro 2 with Evosep One
Software
- MaxQuant quantitative proteomics software package
- Spectronaut
- Perseus framework
- Statistical environment R
- DIA-NN